Product Introduction

NK cell serum-free culture kits MCM CulLymNK-KIT01/02 are specially developed for the efficient expansion of NK cells. The kit chemical composition is clear; KIT01 does not contain any exogenous growth factors and unknown additives. Only a small amount of autologous serum (plasma) can be added during culture to provide a complete and balanced in vitro environment for NK cells expansion. The amplification system used drug-grade antibody to induce T cell apoptosis and specifically activate NK cell proliferation and enhance NK cell killing activity.

Product Advantage

• Stable and safe: medical level factors, component clear, animal origin-free, no trophoblastic layer required.
• High NK purity: 14-day CD3-CD56+ purity > 60%, CD56+ > 70%, CD16+ > 70%.
• Excellent expansion ability: supporting NK cell fast expansion in vitro.
• High killing efficiency: great killing ability towards K562, when E: T=20:1, the killing efficiency can reach 95% when expanded to D14, D21.
• cGMP standard production conditions, using disposable manufacturing techniques to ensure highly stable and consistency of different batches.

Using Method

1.Antibody-coated culture flasks.
2.Isolation of plasma and plasma heat inactivation, and isolation of peripheral blood mononuclear cells (PBMC) by density gradient centrifugation.
3. Antibody-specific activation of NK cells and targeted expansion of NK cells.
4.At the end of the culture, centrifuge to remove the medium and collect the cells

Performance Display

Figure I. NK cell growth curve

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