Preactivated media
Kind and application of preactivated media

• NHS Purose 4 Fast Flow is an NHS (N-hydroxy succinimide) pre-activated agarose matrix. NHS coupling forms a chemically stable amide bond with ligands containing primary amino groups. NHS Purose 4 Fast Flow provides a spacer arm and is therefore particularly suitable for immobilising small protein and peptide ligands.
• ECH Purose 4 Fast Flow is made by covalent linkage of 6-aminohexanoic acid to agarose matrix using an epoxy coupling method. ECH Purose 4 Fast Flow has free carboxyl groups, which are used to couple ligands containing primary amino groups with the carbodiimide coupling method.

• EAH Purose 4 Fast Flow is formed by covalent linkage of 1,6-diaminohexane to agarose matrix using an epoxy coupling method. EAH Purose 4 Fast Flow has free amino groups, which are used to couple ligands containing carboxyl groups with the carbodiimide coupling method.
• Epoxy Purose 4 Fast Flow is an epoxy pre-activated agarose matrix that can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups. Other ligands can be coupled through hydroxyl, amino or thiol groups.

• Bromohydrin Purose 4 Fast Flow is a pre-activated chromatographic medium, its active group can react with amino groups, sulfhydryl groups, hydroxyl groups, etc., and is therefore particularly suitable for immobilizing small protein and peptide ligands.
• SulfoLink Purose 4 Fast Flow is a pre-activated chromatographic medium capable of rapidly binding polypeptides, proteins or other biological ligands containing sulfhydryl groups to form stable thiol-ether bonds with free sulfhydryl groups, thereby fixing ligands to the medium.