Alkali-tolerant Protein A series antibody purification media and prepacked column

iProtein A Purose 4 Fast Flow, iProtein A Purose LX, Rigose MabPureA, and Rigose MabPureA LX are AC media formed on high flow cross-linked agarose matrix and alkali-tolerant protein A-derived ligand. The ligand is the alkali-resistant modified protein A recombined and expressed by E. coli, which allows the use of 0.1-0.5 M sodium hydroxide for CIP. And the modified protein A has enhanced resistance to protease which can achieve lower ligand leakage. So, it is more suitable for the production and application in the downstream purification of antibody.

After long-term using of the medium, too many contaminants may increase the backpressure of the column which can decrease the column efficiency and the binding capacity, even damage the life-time of the medium. Regular CIP cleaning can effectively protect medium. We have a study to verify the work life of the medium by using 0.1 M NaOH with 15 min contact time, combined with binding buffer and low pH elution buffer. Approximately 80% of the initial dynamic binding capacity of Rigose MabPureA LX is retained after 500 cycles with sodium hydroxide.

The binding capacity is one of the important indicators for evaluating purification ability of the medium. Higher binding capacity can improve production efficiency and save production costs. The dynamic binding capacity is a function of the sample residence time and must therefore be defined over a range of different sample residence times. The below figure shows the relation between dynamic binding capacity and residence time for Rigose MabPureA LX and Rigose MabPureA.

Prepacked column