PDE RecTrypsin

Product Introduction

PDE RecTrypsin is a serine protease that selectively hydrolyzes a peptide chain composed of the carboxy terminus of lysine or arginine in a protein. This product is a Recombinant Trypsin powder which is isolated and purified from Recombinant Pichia pastors and then freeze-dried. The product is a biochemical grade genetically engineered enzyme, does not contain excipients and enzyme inhibitors such as DFP, PMSF and TLCK, and has the same activity and specificity as native Trypsin. The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, metal ion chelation.

Product Advantage

Meet Chinese Pharmacopoeia standards.
High safety: recombinant product, animal origin free.
High purity: recombinant product, no other enzymes, host protein and DNA level meet Chinese Pharmacopoeia standards.
Excellent cell digestion ability: high purity, avoid affecting cell growth from impurities.
High S.O.D, same level of digestion with reduced level of added trypsin.

Using Method

• Digestion solution preparation: take the appropriate amount of recombinant trypsin and add DPBS or other balanced salt solution (if EDTA needs to be added, the final concentration is recommended 0-1 mM, preferably no more than 2 mM) to a concentration of 0.1 - 0.3 mg/mL of recombinant trypsin (adjustable as required) and mix gently.
• Filtration and decontamination: the trypsin solution was filtered using a 0.22 μm filter membrane and transferred to a sterile container.
• The filtered trypsin solution can be used directly and the finished product can be stored at 2-8°C for a short period of 2 weeks. -Storage at 20°C for 12 months.