Product Introduction

NK cell serum-free culture kits MCM CulLymNK-KIT01/02 are specially developed for the efficient expansion of NK cells. The kit chemical composition is clear; KIT01 does not contain any exogenous growth factors and unknown additives. Only a small amount of autologous serum (plasma) can be added during culture to provide a complete and balanced in vitro environment for NK cells expansion. The amplification system used drug-grade antibody to induce T cell apoptosis and specifically activate NK cell proliferation and enhance NK cell killing activity.

Product Advantage

Stable and safe: medical level factors, component clear, animal origin-free, no trophoblastic layer required.
High NK purity: 14-day CD3-CD56+ purity > 60%, CD56+ > 70%, CD16+ > 70%.
Excellent expansion ability: supporting NK cell fast expansion in vitro.
High killing efficiency: great killing ability towards K562, when E: T=20:1, the killing efficiency can reach 95% when expanded to D14, D21.

cGMP standard production conditions, using disposable manufacturing techniques to ensure highly stable and consistency of different batches.

Using Method

1.Antibody-coated culture flasks.
2.Isolation of plasma and plasma heat inactivation, and isolation of peripheral blood mononuclear cells (PBMC) by density gradient centrifugation.
3. Antibody-specific activation of NK cells and targeted expansion of NK cells.
4.At the end of the culture, centrifuge to remove the medium and collect the cells