Product Introduction

MCM CulMsc-SFM30 culture medium is developed for human umbili- cal derived MSCs, human placenta MSCs, and human adipose MSCs expansion.The kit is component clear, serum-free, animal origin-free and low protein level. It adapts primary MSCs separate and stable passage and maintains MSC differentiation potential (os teo genesis, adipogenesis, and chondrogenesis).

Product Advantage

Serum-free, animal origin-free and low protein level.
Completely support serum-free culture, no serum or serum substitute required, and suitable for bioreactor.
Adapt primary MSCs separate (Enzyme digestion and tissue explant).
Stable passage and maintain MSC differentiation potential.
cGMP standard production conditions, using disposable manufacturing techniques to ensure highly stable and consistency of different batches.

Using Method

1.Culture carrier coating: for different sizes of culture plates/flasks/cell factories, add the appropriate amount of coating solution and incubate for1-2h for coating.
2.Primary isolation culture/cell inoculation: inoculation of tissue blocks/enzyme digested or recovered MSCs on to well coated culture vectors for culture.

3.Cell pass aging culture: Try ps indigestion,cell collection by cent ri fuga tion,and continued pass aging culture according to a certain density.
4.Cell harvesting and freezing of cells: try ps indigestion,cent ri fuga tion to collect cells,freezing at a certain density with lyophilization solution.