CHO series medium--Tobitec FA & Tobitec FB

Product Introduction

Tobitec FA &FB are chemically-defined feeding mediums without animal-derived components, growth factors, polypeptides, hydrolysates and phenol red, containing amino acids, vitamins, glucose, inorganic salts, and trace elements. Our products are highly efficient feeding mediums specially designed for improving CHO cell growth and production, which can satisfy the nutrient needs from high cell density culture and high level expression. Our products are also suitable for different cell lines including CHO-K1, CHO-S, DG44, CHO-ZN, etc, selected by GS system and DFHR system. Using appropriate feeding strategy based on cell strain traits can significantly improve cell growth and expression level and quality of antibody.

Product Advantage

• Animal origin free, protein-free, fully chemically defined culture media products.
• Suitable for high-density suspension culture of CHO cells with strong adaptability and rich nutrients.
• Suitable for bioreactor large-scale scale-up culture processes, supporting batch, fed-batch and perfusion culture modes. Effective support for transfection and subsequent protein expression, ensuring stable and high quality expression of antibodies and proteins.
• Highly quality imported raw materials to formulate the product with high quality and stability.
• cGMP standard production conditions, using continuous needle milling equipment for power medium production to ensure good product consistency of different batches.

Application Case

Application of Tobitec FA&FB in protein drug production

• CCM: CHO -1 CDM08 +Tobitec FA&FB /Commercial Cell Line: CHO-K1
• Process: Fed batch
• Pruduct: PD-1
• PQAs: CQAs and Titer

Comparative testing of glycosylation data of protein production using different culture media